Pretreatment is a critical step in processing lignocellulosic biomass into biofuel and bioproducts and is considered the energy and cost center of the biomass conversion process. Although a large number of pretreatment technologies have been developed, not all the techniques are viable on a commercial scale at this stage. Moreover, the technology choice and process conditions depend highly on the type of biomass and the overall biorefinery scheme. This chapter provides an overview of different pretreatment methods, including their mechanism, important process parameters, current status, and challenges. The opportunities associated with new technologies and approaches are presented. Emphasis is placed on low-severity thermal pretreatment technologies. The chapter also provides a brief discussion of the challenges to achieving the economic viability of the technologies on a commercial scale. © 2023 Elsevier Inc. All rights reserved.